Journal: Journal of Clinical Medicine

Article Title: Sildenafil Citrate Downregulates PDE5A mRNA Expression in Women with Recurrent Pregnancy Loss without Altering Angiogenic Factors—A Preliminary Study

doi: 10.3390/jcm10215086

Figure Lengend Snippet: TaqMan probes used for RT-PCR in determination of the gene expression of selected angiogenic factors.

Article Snippet: Renin binding protein , RENBP , Hs00234138_m1.

Techniques: Expressing, Binding Assay

Journal: OncoTargets and Therapy

Article Title: Exploring Protein Expression Profiles in Lung Cancer Insufficient Microwave Ablation: Implications for Recurrence

doi: 10.2147/OTT.S508577

Figure Lengend Snippet: Differential Expression Proteins

Article Snippet: The primary antibodies used in our study were listed below CTP synthase 1 (CTPS1) (Boster #A06374-2, 1:200), Thymidylate synthetase (TYMS) (Boster #BM5360, 1:100), NME nucleoside diphosphate kinase 3 (NME3) (Proteintech #15136-1-AP, 1:200), Renin binding protein (RENBP) (Bioss #bs-8497R, 1:200), Fucose kinase (FCSK) (Sangon #D127113, 1:300), Pyridoxal phosphatase (PDXP) (Abclonal #A17455, 1:300), and Sulfite oxidase (SUOX) (Proteintech #15075-1-AP, 1:200).

Techniques: Expressing, Binding Assay

Journal: OncoTargets and Therapy

Article Title: Exploring Protein Expression Profiles in Lung Cancer Insufficient Microwave Ablation: Implications for Recurrence

doi: 10.2147/OTT.S508577

Figure Lengend Snippet: Relationship Between DEPs and Prognosis From TCGA Dataset

Article Snippet: The primary antibodies used in our study were listed below CTP synthase 1 (CTPS1) (Boster #A06374-2, 1:200), Thymidylate synthetase (TYMS) (Boster #BM5360, 1:100), NME nucleoside diphosphate kinase 3 (NME3) (Proteintech #15136-1-AP, 1:200), Renin binding protein (RENBP) (Bioss #bs-8497R, 1:200), Fucose kinase (FCSK) (Sangon #D127113, 1:300), Pyridoxal phosphatase (PDXP) (Abclonal #A17455, 1:300), and Sulfite oxidase (SUOX) (Proteintech #15075-1-AP, 1:200).

Techniques:

Journal: International Journal of Nephrology

Article Title: Effect of the Direct Renin Inhibitor Aliskiren on Urinary Albumin Excretion in Spontaneous Type 2 Diabetic KK- A y Mouse

doi: 10.1155/2013/519130

Figure Lengend Snippet: Expression of MMP-2, MMP-9, TIMP-1, TIMP-2, fibronectin, type IV collagen, MCP-1, and (P)RR in the kidneys of each mouse using real-time PCR (A: untreated KK mice at 8 weeks of age, B: untreated KK mice at 12 weeks of age, C: untreated KK- A y mice at 8 weeks of age, D: untreated KK- A y mice at 12 weeks of age, and E: treated KK- A y mice at 12 weeks of age). The ratio of MMP-2 (a), MMP-9 (b), TIMP-1 (c), TIMP-2 (d), fibronectin (e), type IV collagen (f), MCP-1 (g), and (P)RR (h) mRNAs to GAPDH mRNA is shown in the kidneys of mice from each group. These expressions were increased significantly in group 4 compared with groups 1, 2, and 3. This increase was attenuated in group 5 (* P < 0.001 versus untreated KK- A y mice at 12 weeks of age).

Article Snippet: To measure gene expression in each tissue fraction, real-time PCR was performed using primers supplied with the commercially available assays obtained from Applied Biosystems (MMP-2: Mm01253624 _m1, MMP-9: Mm00600163 _m1, TIMP-1: Mm01341361 _m1, TIMP-2: Mm00441825 _m1, Fibronectin: Mm01256744 _m1, Type IV collagen: Mm01210125 _m1, MCP-1: Mm00441242 _m1, (Pro) renin receptor ((P)RR): Mm00510396 _m1, and Glyceraldehyde 3-phosphate dehydrogenase (GAPDH): Mm99999915 _g1).

Techniques: Expressing, Real-time Polymerase Chain Reaction

Journal: International Journal of Nephrology

Article Title: Effect of the Direct Renin Inhibitor Aliskiren on Urinary Albumin Excretion in Spontaneous Type 2 Diabetic KK- A y Mouse

doi: 10.1155/2013/519130

Figure Lengend Snippet: Immunohistochemical staining of MMP-2, MMP-9, TIMP-1, and TIMP-2 in the renal sections of each mouse (O: untreated KK- A y mice at 12 weeks of age without primary Ab, A: untreated KK mice at 8 weeks of age, B: untreated KK mice at 12 weeks of age, C: untreated KK- A y mice at 8 weeks of age, D: untreated KK- A y mice at 12 weeks of age, and E: treated KK- A y mice at 12 weeks of age). Stainings showing the expression of MMP-2 (a), MMP-9 (b), TIMP-1 (c), and TIMP-2 (d) in the kidneys of mice from each group. These expressions were increased significantly in group 4 compared with groups 1, 2, and 3. This increase was attenuated in group 5 (* P < 0.001 versus untreated KK- A y mice at 12 weeks of age). Images were taken at 400-fold (a and d) and 200-fold (b and c) magnification. WGA indicates whole glomerular area. WIA indicates whole interstitial area.

Article Snippet: To measure gene expression in each tissue fraction, real-time PCR was performed using primers supplied with the commercially available assays obtained from Applied Biosystems (MMP-2: Mm01253624 _m1, MMP-9: Mm00600163 _m1, TIMP-1: Mm01341361 _m1, TIMP-2: Mm00441825 _m1, Fibronectin: Mm01256744 _m1, Type IV collagen: Mm01210125 _m1, MCP-1: Mm00441242 _m1, (Pro) renin receptor ((P)RR): Mm00510396 _m1, and Glyceraldehyde 3-phosphate dehydrogenase (GAPDH): Mm99999915 _g1).

Techniques: Immunohistochemical staining, Staining, Expressing

Journal: Kidney & blood pressure research

Article Title: The Role of β-Adrenergic Overstimulation in the Early Stages of Renal Injury.

doi: 10.1159/000485931

Figure Lengend Snippet: Fig. 7. Quantitative PCR analysis of renal cortex from the control or ISO treated rats. The mRNA expression levels of angiotensinogen - Agt, renin - Ren, angiotensin I converting enzyme - ACE 1 (A) are shown. Representative photomicrographs of the renal cortex (B and C) or medulla (D and E), illustrating semiquantitative analysis of AGT, ACE1 and REN, and immunostaining of cortical ACE1 and medullar renin. Kidney sections (4-µm thick) were stained using immunohistochemical method. Standard images were captured using a morphometric program (NIS-Element) with a x 20 objective. Also shown magnifications of positive-stained proximal tubules (PT) for angiotensinogen and ACE1 and collecting duct (CD) for renin from control and ISO treated rats. Values are means ± SE (n = 6-8). All qPCR samples were assayed in duplicate.

Article Snippet: As previously described [25] and summarized here, kidney sections (4-μm thick) were deparaffinized and nonspecific protein binding was blocked by incubation with 10% goat serum in PBS for 60 min. Then, kidney sections were incubated overnight at 4oC with specific primary antibodies [rabbit polyclonal to renin (1:200, Biorbyt, San Francisco, CA, USA), mouse polyclonal to angiotensinogen (1:200, Biorbyt); rabbit angiotensin converting enzyme 1 (1:75, Novus Biologicals, Littleton, CO); rabbit NADPH oxidase 4 (1:200, Abcam, Cambridge, MA); rabbit eIF2 alpha (1:150, Cell Signaling, Denver, MA); rabbit IkB alpha (1:100, Abcam); rabbit NFκB1 p65 (1:75, Santa Cruz Biotechnology, Dallas, TX, USA); rabbit IL-1 beta (1:100, Santa Cruz Biotechnology); rabbit Bax (1:75, Santa Cruz Biotechnology), rabbit Bcl-2 (1:75, Cell Signaling, Denver, MA, USA] and subsequently with specific ant- rabbit or anti-mouse secondary antibodies for two hours at room temperature.

Techniques: Real-time Polymerase Chain Reaction, Control, Expressing, Immunostaining, Staining, Immunohistochemical staining

Journal: BioMed Research International

Article Title: Elucidation of the Genomic-Epigenomic Interaction Landscape of Aggressive Prostate Cancer

doi: 10.1155/2021/6641429

Figure Lengend Snippet: List of the 41 genes containing germline, somatic, and epigenetic mutations transcriptionally associated with aggressive PCa discovered through analysis and integration of multi-omics data.

Article Snippet: TTC7A , rs10194115 , 5.00 E − 07 , cg04574383 , 1.24 E − 14 , 5 , 3.66 E − 10 , 1.

Techniques: Biomarker Discovery, Mutagenesis